23 August 2016
La Guaira – Emelec
Referee: Roberto Tobar (CHI, photo)
Assistant Referee 1: Raúl Orellana (CHI)
Assistant Referee 2: José Retamal (CHI)
Fourth Official: Patricio Polic (CHI)
Referee Assessor: Adrián Gómez (VEN)
Banfield – San Lorenzo
Referee: Luiz De Oliveira (BRA)
Assistant Referee 1: Rodrigo Correa (BRA)
Assistant Referee 2: Guilherme Dias (BRA)
Fourth Official: Dewson Freitas (BRA)
Referee Assessor: Carlos Coradina (ARG)
Blooming – Junior
Referee: Jonathan Fuentes (URU)
Assistant Referee 1: Miguel Nievas (URU)
Assistant Referee 2: Richard Trinidad (URU)
Fourth Official: Leodán Gonzalez (URU)
Referee Assessor: Pedro Saucedo (BOL)
24 August 2016
Real Garcilaso – Palestino
Referee: Raúl Orosco (BOL)
Assistant Referee 1: Juan Montaño (BOL)
Assistant Referee 2: José Antelo (BOL)
Fourth Official: José Jordan (BOL)
Referee Assessor: Ana Pérez (PER)
Cerro Porteño – Real Potosí
Referee: Diego Haro (PER)
Assistant Referee 1: Jorge Yupanqui (PER)
Assistant Referee 2: Braulio Cornejo (PER)
Fourth Official: Joel Alarcón (PER)
Referee Assessor: Manuel Bernal (PAR)
Bolivar – Atlético Nacional
Referee: Enrique Cáceres (PAR)
Assistant Referee 1: Juan Zorilla (PAR)
Assistant Referee 2: Roberto Cañete (PAR)
Fourth Official: José Mendez (PAR)
Referee Assessor: Freddy Vilty (BOL)
Independiente Medellín – Sportivo Luqueño
Referee: Roddy Zambrano (ECU)
Assistant Referee 1: Christian Lescano (ECU)
Assistant Referee 2: Byron Romero (ECU)
Fourth Official: Diego Lara (ECU)
Referee Assessor: Otalvaro Polanco (COL)
Estudiantes – Belgrano
Referee: Julio Quintana (PAR)
Assistant Referee 1: Eduardo Cardozo (PAR)
Assistant Referee 2: Milciades Saldivar (PAR)
Fourth Official: Eber Aquino (PAR)
Referee Assessor: Rodolfo Otero (ARG)
Figueirense – Flamengo
Referee: Daniel Fedorczuk (URU)
Assistant Referee 1: Mauricio Espinosa (URU)
Assistant Referee 2: Nicolás Tarán (URU)
Fourth Official: Óscar Rojas (URU)
Referee Assessor: Nilson Moncao (BRA)
Santa Cruz – Sport Recife
Referee: Julio Bascuñán (CHI)
Assistant Referee 1: Marcelo Barraza (CHI)
Assistant Referee 2: Christian Schiemann (CHI)
Fourth Official: Eduardo Gamboa (CHI)
Referee Assessor: Milton Otaviano (BRA)
25 August 2016
Cuiabá – Chapecoense
Referee: Jorge Baliño (ARG)
Assistant Referee 1: Ezequiel Brailovksy (ARG)
Assistant Referee 2: Christian Navarro (ARG)
Fourth Official: Fernando Rapallini (ARG)
Referee Assessor: Alicio Peña (BRA)
Lanús – Independiente
Referee: Wilmar Roldán (COL)
Assistant Referee 1: Humberto Clavijo (COL)
Assistant Referee 2: John León (COL)
Fourth Official: Juan Pontón (COL)
Referee Assessor: Luis Pasturenzi (ARG)
Zamora – Wanderers
Referee: Leandro Vuaden (BRA)
Assistant Referee 1: Bruno Boschilia (BRA)
Assistant Referee 2: Bruno Pires (BRA)
Fourth Official: Pericles Cortez (BRA)
Referee Assessor: Bernardo Corujo (VEN)
Sol de América – Sport Huancayo
Referee: Germán Delifno (ARG)
Assistant Referee 1: Diego Bonfa (ARG)
Assistant Referee 2: Lucas Germanotta (ARG)
Fourth Official: Fernando Espinoza (ARG)
Referee Assessor: Joel Ruiz (PAR)
Vitoria – Coritiba
Referee: José Argote (VEN)
Assistant Referee 1: Francheskoly Chacón (VEN)
Assistant Referee 2: Elbis Gómez (VEN)
Fourth Official: Marlon Escalante (VEN)
Referee Assessor: Manoel Serapiao (BRA)