23 August 2016
Alianza FC – Antigua GFC
Referee: John Pitti (PAN, photo)
Assistant Referee 1: Gabriel Victoria (PAN)
Assistant Referee 2: Christian Ramirez (HON)
Fourth Official: Oscar Davila (NCA)
Vancouver Whitecaps – Sporting KC
Referee: Erick Miranda (MEX)
Assistant Referee 1: Alberto Morin (MEX)
Assistant Referee 2: Andres Hernandez (MEX)
Fourth Official: Roberto Garcia (MEX)
Pachuca – Police United
Referee: Armando Villarreal (USA)
Assistant Referee 1: Corey Parker (USA)
Assistant Referee 2: Frank Anderson (USA)
Fourth Official: Mark Geiger (USA)
24 August 2016
Don Bosco – Arabe Unido
Referee: Sandy Vasquez (DOM)
Assistant Referee 1: Walter Lopez (HON)
Assistant Referee 2: Walter Cid (DOM)
Fourth Official: Hector Rodriguez (HON)
Tigres – Plaza Amador
Referee: Joel Aguilar (SLV)
Assistant Referee 1: Jose Mangandi (SLV)
Assistant Referee 2: David Moran (SLV)
Fourth Official: Jonathan Polanco (GUA)
Real Esteli – FC Dallas
Referee: Jaime Herrera (SLV)
Assistant Referee 1: Juan Zumba (SLV)
Assistant Referee 2: William Torres (SLV)
Fourth Official: Henry Bejarano (CRC)
25 August 2016
CD Dragon – Saprissa
Referee: Fernando Guerrero (MEX)
Assistant Referee 1: Marcos Quintero (MEX)
Assistant Referee 2: Salvador Rodriguez (MEX)
Fourth Official: Jose Penaloza (MEX)
W Connection – H Progreso
Referee: Baldomero Toledo (USA)