13 September 2016
Emelec – La Guaira
Referee: Wilmar Roldan (COL, photo)
Assistant Referee 1: Eduardo Diaz (COL)
Assistant Referee 2: Alexander Leon (COL)
Fourth Official: Adrian Velez (COL)
Referee Assessor: Juan Corozo (ECU)
San Lorenzo – Banfield
Referee: Wilton Sampaio (BRA)
Assistant Referee 1: Kleber Gil (BRA)
Assistant Referee 2: Bruno Boschilia (BRA)
Fourth Official: Pericles Cortez (BRA)
Referee Assessor: Ricardo Casas (ARG)
Wanderers – Zamora
Referee: Dario Herrera (ARG)
Assistant Referee 1: Ariel Scime (ARG)
Assistant Referee 2: Diego Bonfa (ARG)
Fourth Official: Fernando Espinoza (ARG)
Referee Assessor: Martin Vazquez (URU)
Atletico National – Bolivar
Referee: Roddy Zambrano (ECU)
Assistant Referee 1: Edwin Bravo (ECU)
Assistant Referee 2: Flavio Nall (ECU)
Fourth Official: Omar Ponce (ECU)
Referee Assessor: Otalvaro Polanco (COL)
Coritiba – Vitoria
Referee: Carlos Orbe (ECU)
Assistant Referee 1: Luis Vera (ECU)
Assistant Referee 2: Juan Macias (ECU)
Fourth Official: Juan Albarracin (ECU)
Referee Assessor: Ednilson Corona (BRA)
Flamengo – Figueirense
Referee: Wilson Lamouroux (COL)
Assistant Referee 1: Alexander Guzman (COL)
Assistant Referee 2: Dionisio Ruiz (COL)
Fourth Official: Gustavo Murillo (COL)
Referee Assessor: Ana Paula Oliveira (BRA)
Sport Recife – Santa Cruz
Referee: Diego Haro (PER)
Assistant Referee 1: Raul Lopes (PER)
Assistant Referee 2: Michael Orue (PER)
Fourth Official: Henry Gambetta (PER)
Referee Assessor: Antonio Pereira (BRA)
Chapecoense – Cuiaba
Referee: Jesus Valenzuela (VEN)
Assistant Referee 1: Luis Murillo (VEN)
Assistant Referee 2: Tulio Moreno (VEN)
Fourth Official: Adrian Cabello (VEN)
Referee Assessor: Roberto Perassi (BRA)
14 September 2016
Independiente – Lanus
Referee: Enrique Caceres (PAR)
Assistant Referee 1: Juan Zorrilla (PAR)
Assistant Referee 2: Dario Gaona (PAR)
Fourth Official: Eber Aquino (PAR)
Referee Assessor: Rodolfo Otero (ARG)
Junior – Blooming
Referee: Raphael Claus (BRA)
Assistant Referee 1: Alessandro Rocha (BRA)
Assistant Referee 2: Bruno Pires (BRA)
Fourth Official: Luiz De Oliveira (BRA)
Referee Assessor: Pablo Montoya (COL)
15 September 2016
Huancayo – Sol de America
Referee: Nestor Pitana (ARG)
Assistant Referee 1: Juan Belatti (ARG)
Assistant Referee 2: Cristian Navarro (ARG)
Fourth Official: Diego Abal (ARG)
Referee Assessor: Cesar Escano (PER)
Real Potosí – Cerro Porteno
Referee: Oscar Rojas (URU)
Assistant Referee 1: Carlos Pastorino (URU)
Assistant Referee 2: Gabriel Popovits (URU)
Fourth Official: Esteban Ostojich (URU)
Referee Assessor: Ivan Gamboa (BOL)
Palestino – Real Garcilaso
Referee: Mario Diaz De Vivar (PAR)
Assistant Referee 1: Rodney Aquino (PAR)
Assistant Referee 2: Carlos Caceres (PAR)
Fourth Official: Jose Mendez (PAR)
Referee Assessor: Ivan Guerrero (CHI)
Luqueno – Medellin
Referee: Roberto Tobar (CHI)
Assistant Referee 1: Christian Schiemann (CHI)
Assistant Referee 2: Marcelo Barazza (CHI)
Fourth Official: Eduardo Gamboa (CHI)
Referee Assessor: Ubaldo Aquino (PAR)
Belgrano – Estudiantes
Referee: Victor Carrillo (PER)
Assistant Referee 1: Coty Carrera (PER)
Assistant Referee 2: Jorge Yupanqui (PER)
Fourth Official: Michael Espinoza (PER)
Referee Assessor: Carlos Coradina (ARG)