16 August 2016Referee: Henry Bejarano (CRC, photo)
Assistant Referee 1: Osvaldo Luna (CRC)
Assistant Referee 2: Warner Castro (CRC)
Fourth Official: Ricardo Montero (CRC)
Alianza FC – New York Red Bulls
Referee: Oscar Moncada (HON)
Assistant Referee 1: Oscar Velazquez (HON)
Assistant Referee 2: Omar Leiva (HON)
Fourth Official: Raul Castro (HON)
Olimpia – Police United
Referee: Jonathan Polanco (GUA)
Assistant Referee 1: Ronaldo De La Cruz (GUA)
Assistant Referee 2: Geovany Garcia (SLV)
Fourth Official: Joel Aguilar (SLV)
17 August 2016
Monterrey – Arabe Unido
Referee: Mario Escobar (GUA)
Assistant Referee 1: Marco Diaz (GUA)
Assistant Referee 2: Rene Ochoa (GUA)
Fourth Official: Oscar Reyna (GUA)
Herediano – Tigres
Referee: Marlon Mejia (SLV)
Assistant Referee 1: William Torres (SLV)
Assistant Referee 2: Douglas Bermudez (SLV)
Fourth Official: Jaime Herrera (SLV)
18 August 2016
Suchitepequez – Real Esteli
Referee: Ricangel DeLeca (ARU)
Assistant Referee 1: Richard Washington (JAM)
Assistant Referee 2: Eugene Farro (ARU)
Fourth Official: Valdin Legister (JAM)
Pumas – Progreso
Referee: Jose Kellys (PAN)
Assistant Referee 1: Hermenerito Leal (GUA)
Assistant Referee 2: Henry Pupiro (NCA)
Fourth Official: John Pitti (PAN)
Saprissa – Dragon
Referee: Mathieu Bourdeau (CAN)
Assistant Referee 1: Philippe Briere (CAN)
Assistant Referee 2: Mark Hurd (USA)
Fourth Official: David Gantar (CAN)